Lynne Margaret Dieckman, MS, PhD
Associate Professor
Contact
College of Arts and Sciences
Chemistry
Chemistry Faculty
Biochemistry
RGSB - Rigge Science Building - 309B
Lynne Margaret Dieckman, MS, PhD
Associate Professor
Research Focus
Research in the Dieckman lab focuses on the mechanisms by which errors in DNA replication and repair and chromatin organization lead to genome instability and disease. Specifically, our main goal is to characterize the interactions of key factors that are essential for the interplay between genomic and epigenetic stability. Two of these factors include the proteins PCNA (the processivity factor that stimulates DNA polymerases during DNA replication and repair) and CAF-1 (the main histone chaperone that is necessary for packaging DNA into nucleosomes following replication and repair). The interaction between PCNA and CAF-1 is crucial for proper DNA synthesis, assembly of nucleosomes, and preservation of epigenetic marks. Students will learn biochemical techniques used in modern biomedical research in order to determine the mechanistic basis of the network of interactions between CAF-1, PCNA, DNA, and other factors during replication-dependent nucleosome assembly and to work towards an understanding of proper DNA maintenance.
Department
Chemistry and Biochemistry
Position
Associate Professor
Books
- Pryor J. M., etal Eukaryotic Y-Family Polymerases: A Biochemical and Structural Perspective [Book Chapter] 2014
Publications
- Journal of molecular biology
Orndorff Keely S., etal Structural Basis for the Interaction Between Yeast Chromatin Assembly Factor 1 and Proliferating Cell Nuclear Antigen, p. 168695 2024 - ACS omega
Kamayirese Seraphine, etal Optimizing Phosphopeptide Structures That Target 14-3-3ε in Cutaneous Squamous Cell Carcinoma 2024 - Progress in Biophysics and Molecular Biology
Dieckman Lynne, Something's gotta give
163, p. 46 - 59 2021 - Journal of Biological Chemistry
Shaw Jeff A., etal Salmonella enterica serovar Typhimurium has three transketolase enzymes contributing to the pentose phosphate pathway
293:29, p. 11271 - 11282 2018 - PloS one
Kondratick Christine M., etal Crystal structures of PCNA mutant proteins defective in gene silencing suggest a novel interaction site on the front face of the PCNA ring
13:3 2018 - PloS One
Kondratick C. M., etal Identification of New Mutations at the PCNA Subunit Interface that Block Translesion Synthesis
11:6, p. (12 pages) 2016 - Biochemistry
Dieckman Lynne M., etal Distinct structural alterations in proliferating cell nuclear antigen block DNA mismatch repair
52:33, p. 5611 - 5619 2013 - DNA Repair
Dieckman Lynne M., etal PCNA trimer instability inhibits translesion synthesis by DNA polymerase η and by DNA polymerase δ
12:5, p. 367 - 376 2013 - Subcellular Biochemistry
Dieckman Lynne M., etal PCNA structure and function
62, p. 281 - 299 2012 - Biochemistry
Dieckman Lynne M., etal Pre-steady state kinetic studies of the fidelity of nucleotide incorporation by yeast DNA polymerase δ
49:34, p. 7344 - 7350 2010
Grants
CAREER: Understanding Protein Interactions that Link DNA Replication and Nucleosome Assembly/Sponsor: National Science Foundation/NSF
INBRE: Structure and Function of Complexes That Regulate Gene Silencing/Sponsor: University of Nebraska Medical Center/UNMC
INBRE: Mechanisms of Protein-Protein Interactions that Regulate Nucleosome Assembly/Sponsor: University of Nebraska Medical Center/UNMC
Identifying B Cells and B Cell Receptor Genes that Bind the Autoantigen PCNA/Sponsor: Dr. George F. Haddix President's Faculty Research Grant