Michael G . Nichols, MS, PhD

Department

Physics

Position

Professor

Books

• Auditory and Vestibular Research : Methods and Protocols (Methods in Molecular Biology, Vol. 1427)
  Nichols Michael G., Hallworth Richard J., The Single-Molecule Approach to Membrane Protein Stoichiometry [Book Chapter] 2016
• Reviews in Fluorescence 2009
  Anderson Jon P., Grone Daniel L., Williams John G., Nichols Michael G., Metal Enhancement of Near-IR Fluorescence for Molecular Biotechnology Applications [Book Chapter] 2011
• Auditory and Vestibular Research : Methods and Protocols
  Jensen-Smith Heather, Currall Benjamin, Rossino Danielle, Tiede Leann, Nichols Michael, Hallworth Richard, Fluorescence microscopy methods in the study of protein structure and function [Book Chapter] 2009

Publications

• Review of scientific instruments
  Aboyewa O. B., Akinleye H. B., Wrubel J. P., Nichols M. G., Design of a simple radio frequency circuit for implementing the open-ended coaxial probe method for permittivity measurement
  93:11, p. 114705 - 114705 2022
• Biophysical journal
  Nichols Michael G., Farr Tyler B., Hubbs Hayden M., Kalhorn Connor J., Myers Cecilia M., Nguyen Alicia C., Rogers Samuel J., Snyder Dan R., Tran Thien, Varghese George J., Wood Daniel, Pham Dan L., Hansen Laura A., Impact of cell culture conditions on assessment of cellular metabolism in vitro by NAD(P)H phasor flim with application to in vivo diagnostic imaging for non-invasive biopsy
  121:3, p. 276a - 276a 2022
• Journal of biomedical optics
  Pham Dan L, Nichols Michael G, Miller Christina R, Myers Molly S, Myers Dominick M, Hansen Laura A, Development and characterization of phasor-based analysis for FLIM to evaluate the metabolic and epigenetic impact of HER2 inhibition on Squamous Cell Carcinoma (SCC) cultures
  26:10 2021
• Journal of Biomedical Optics
  Pham Dan L., Myers Dominick M., Miller Christina R., Myers Molly S., Hansen Laura A., Nichols Michael G., Development and characterization of phasor-based analysis for FLIM to evaluate the metabolic and epigenetic impact of HER2 inhibition on squamous cell carcinoma cultures
  26:10 2021
• ACS Omega
  Bony Badrul Alam, Gee Connor C., Kievit Forrest M., Miller Hunter A., Tarudji Aria W., Sarella Anandakumar, Nichols Michael G., Ultrasmall Mixed Eu-Gd Oxide Nanoparticles for Multimodal Fluorescence and Magnetic Resonance Imaging of Passive Accumulation and Retention in TBI
  5:26, p. 16220 - 16227 2020
• Journal of Drug Targeting
  Meerovich Igor, Nichols Michael G., Dash Alekha K., Low-intensity light-induced drug release from a dual delivery system comprising of a drug loaded liposome and a photosensitive conjugate
  28:6, p. 655 - 667 2020
• Biophysical journal
  Nichols Michael G., Hansen Laura A., Huerter Ben G., Myers Molly, Myers Dominick, Jackson Kelsey A., Che Dan L., Altuzar Emiliano, Long Anya, Morris Jackson W., Sotelo Katie D., Nguyen Duyen, Kalhorn Connor J., Varghese George, Frances Gabriel Marifel, Phasor-FLIM Quantification of Changes in Keratinocyte Metabolism and Tissue Architecture in a Longitudinal Study of UV-induced Skin Cancer
  118:3, p. 307a - 307a 2020
• Journal of Drug Targeting
  Meerovich Igor, Nichols Michael G., Dash Alekha K., Low-intensity light-induced paclitaxel release from lipid-based nano-delivery systems
  27:9, p. 971 - 983 2019
• Journal of Biomedical Optics
  Desa Danielle E., Nichols Michael G., Smith Heather Jensen, Aminoglycosides rapidly inhibit NAD(P)H metabolism increasing reactive oxygen species and cochlear cell demise
  24:5 2019
• Biophysical journal
  Myers Dominick, Nichols Michael G., Yang Brooke L., Sotelo Katie D., Huerter Ben G., Che Dan L., Myers Molly, Nguyen Duyen, Hansen Laura A., Frances Gabriel Marifel, Jackson Kelsey A., Two-Photon Imaging of NADH in SKH1 Mice Reveals Changes in Keratinocyte Metabolism with Chronic UVA Exposure
  116:3, p. 281a - 282a 2019
• Biophysical journal
  Nichols Michael G., Gabriel Marifel F., Mills Alexis M., Myers Dominick M., Pham Dan L., Pociupany Inga N., Sotelo Katie D., Troup Kathleen E., Das Rohan, Miller Christina R., Metabolic Imaging of Squamous Cell Carcinoma Cells and In Vivo Skin by Phasor FLIM
  112:3, p. 298 - 299a 2017
• Biophysical Journal
  Prathivadhi-Bhayankaram Sruti V., Nichols Michael, Guck Jochen, Ekpenyong Andrew E., Ning Jianhao, Mimlitz Michael, Gross Erin, Taylor Carolyn, Chemotherapy impedes in vitro microcirculation and promotes migration of leukemic cells with impact on metastasis
  112:3, p. 124a - 124a 2017
• Biophysical journal
  Prathivadhi-Bhayankaram Sruti V., Taylor Carolyn E., Ning Jianhao, Nichols Michael, Ekpenyong Andrew E., Cell Mechanical Properties and Cancer Metastasis: Effects of Cancer Drugs and Radiotherapy
  110:3, p. 621a - 621a 2016
• Biochemical and Biophysical Research Communications
  Prathivadhi-Bhayankaram Sruti, Ning Jianhao, Mimlitz Michael, Taylor Carolyn, Gross Erin M., Nichols Michael G., Guck Jochen, Ekpenyong Andrew E., Chemotherapy Impedes in Vitro Microcirculation and Promotes Migration of Leukemic Cells with Impact on Metastasis
  479:4, p. 841 - 846 2016
• Journal of Biomedical Optics
  Zholudeva L. V., Ward Kristina G., Nichols Michael G., Smith H. J., Jensen Smith Heather C., Gentamicin differentially alters cellular metabolism of cochlear hair cells as revealed by NAD(P)H fluorescence lifetime imaging
  20:5, p. (13 pages) 2015
• The FASEB journal
  Cerutis D. Roselyn, Nichols Michael, McVaney Timothy, Hironaka Michael, Miyamoto Takanari, Khan Shakeel, Ogunleye Afolabi, Complementing Confocal Detection of Antibody‐labeled Lysophosphatidic Acid Receptors in Human Gingivae with Label‐free Second Harmonic Generation Confocal Microscopy Detection of Collagen
  29:S1 2015
• Biophysical journal
  Desa Danielle, Nichols Michael G., Jensen Smith Heather, The Role of Complex I in Mitochondrial Reactive Oxygen Species Formation in Cochlear Sensory and Supporting Cells during Ototoxic Aminoglycoside Exposure
  108:2, p. 611a - 611a 2015
• Biophysical journal
  Miller Christina R., Nichols Michael G., Metabolic Profiling of the Skin to Monitor the Onset and Progression of Squamous Cell Carcinoma through Time- and Wavelength-Resolved Fluorescence Lifetime Imaging
  108:2, p. 478 - 478a 2015
• The FASEB journal
  Cerutis D. Roselyn, Miyamoto Takanari, Nichols Michael, Jenzer Andrew, Khan Shakeel, McVaney Timothy, Kaldahl Wayne, Label‐free imaging of the regenerating human periodontal ligament and gingival tissue using second harmonic generation confocal microscopy (LB41)
  28:S1, p. - n/a 2014
• Biophysical journal
  Smith Heather Jensen, Desa Danielle, Miller Christina, Nichols Michael G., Endogenous Differences in Cochlear Sensory and Supporting Cell Mitochondrial Metabolism Bias Free Radical Production during Ototoxin Exposure
  106:2, p. 185a - 185a 2014
• Biophysical journal
  Zholudeva Lyandysha V., Ward Kristina G., Nichols Michael G., Smith Heather Jensen, Two-Photon Fluorescence Lifetime Imaging for Metabolic Profiling of Cochlear Dysfunction
  106:2, p. 185a - 185a 2014
• Biophysical Journal
  Holman Nathan, Hauger Eric, Nichols Michael, Sidebottom David, Haas Eric, One and Two Photon Fluorescence Correlation Spectroscopy on Proteins in Glucose Solutions
  104:2, p. 346a - 347a 2013
• Microscopy and Microanalysis
  Hallworth Richard J., Nichols Michael G., Stark Kelsey, Zholudeva Lyandysha V., Currall Benjamin B., The Conserved Tetrameric Subunit Stoichiometry of Slc26 Proteins
  19:4, p. 799 - 807 2013
• Biophysical Journal
  Nichols Michael G., Zholudeva Lyandysha V., Desa Danielle E., Meyer Christian T., Lehnerz Marcus J., Metabolic Profiling of Multicell Tumor Spheroids by NADH Fluorescence and Spatially-Resolved Oximetry
  104:2, Supplement 1, p. 304a 2013
• Biophysical Journal
  Ward Kristina G., Jensen Smith Heather C., Zholudeva Lyandysha V., Nichols Michael G., Gentamiacin Differentially Affects OHC and IHC Metabolism as Revealed by NADH Fluorescence Lifetime Imaging
  104:2, Supplement 1, p. 305a 2013
• Hallworth Richard J., Nichols Michael G., Introduction 2012
• PLoS One
  Jensen-Smith H. C., Jensen Smith Heather C., Hallworth Richard J., Nichols Michael G., Gentamicin rapidly inhibits mitochondrial metabolism in high-frequency cochlear outer hair cells.
  7:6, p. e38471 2012
• Journal of Neurophysiology
  Hallworth Richard J., Nichols Michael G., Prestin in HEK cells is an obligate tetramer
  107:1, p. 5 - 11 2012
• Microscopy and Microanalysis
  Vergen Jorge, Hecht Clifford, Zholudeva Lyandysha V., Marquardt Meg, Nichols Michael G., Hallworth Richard J., Metabolic Imaging Using Two-Photon Excited NADH Intensity and Fluorescence Lifetime Imaging
  18:4, p. 761 - 770 2012
• Microscopy and Microanalysis
  Hallworth Richard J., Nichols Michael G., Single Molecule Imaging Approach to Membrane Protein Stoichiometry
  18:4, p. 771 - 780 2012
• Nebraska Academy of Sciences, PO Box 880410 Lincoln NE 68588-0410 United States. [np]. Jun 2011
  Ward Kristina, Nichols Michael, Ramsey Lonzale, DEVELOPMENT OF A FREQUENCY-DOMAIN PHASE-SENSITIVE FLUORIMETER TO QUANTIFY THE OXYGEN CONSUMPTION OF LIVING CELLS 2011
• Biophysical journal
  Nichols Michael G., Hecht Clifford S., Tiede LeAnn M., Ramsey Lonzale, Vergen Jorge A., Ward Kristina G., Hallworth Richard, Assessing the Metabolic State of Cultured Cells and the Intact Cochlea by Two-Photon Fluorescence Intensity and Lifetime Imaging
  100:3, p. 319 - 319a 2011
• Biophysical journal
  Thomen Robert P., Woldemariam Semere M., Nichols Michael G., Single Cell Refractive Index Measurements for Optical Biomechanics
  100:3, p. 141 - 141a 2011
• Biophysical journal
  Tonniges Jeff, Fritzsch Bernd, Hansen Maria, Duncan Jeremy, Gray Brian, Nichols Michael G., Easwaran Arunkumar, Diffusion Measurements of Lipophilic Fluorescent Probes in Fixed Tissue and Living Cells
  98:3, p. 397a - 397a 2010
• Biophysical journal
  Nichols Michael G., Burkart Anya K., Chaput Joy L., Thomen Robert P., Ward Kristina G., Woldemariam Semere M., A Comparison of Single-Cell Elasticity of Osteogenic Cells Measured with the Optical Stretcher and Holographic Microscope
  98:3, p. 369 - 369a 2010
• Journal of Microscopy
  Tonniges J., Hansen M., Duncan J., Bassett M. J., Fritzsch B., Gray B. D., Easwaran A., Nichols Michael G., Photo- and bio-physical characterization of novel violet and near-infrared lipophilic fluorophores for neuronal tracing
  239:2, p. 117 - 134 2010
• Brain Research
  Tiede LeAnn, Steyger Peter S., Nichols Michael G., Hallworth Richard J., Metabolic imaging of the organ of corti — A window on cochlea bioenergetics
  1277, p. 37 - 41 2009
• Nebraska Academy of Sciences, PO Box 880410 Lincoln NE 68588-0410 United States. [np]. Apr 2009
  Smith Timothy, Nichols Michael, CELL SIGNALLING AND THE OPTICAL STRETCHER 2009
• Nebraska Academy of Sciences, PO Box 880410 Lincoln NE 68588-0410 United States. [np]. Apr 2009
  Vergen Jorge, LeAnn Leann, Hecht Clifford, Nichols Michael, COMPARISON OF TWO-PHOTON NADH INTENSITY IMAGING AND FLUORESCENCE LIFETIME IMAGING TO QUANTIFY CELLULAR ENERGETICS 2009
• Biophysical journal
  Hansen Maria, Nichols Michael, Bassett Matthew, Fritzsch Bernd, Gray Brian, Tonniges Jeff, Duncan Jeremy, Diffusion-Matched and Spectrally-Discrete Lipophilic Probes for Neuronal Tracing
  96:3, p. 151a - 151a 2009
• Biophysical journal
  Tiede LeAnn M., Hallworth Richard, Vergen Jorge A., Hecht Clifford, Nichols Michael G., Using Two-photon Excited Fluorescence Intensity- and Lifetime-based NADH Imaging to Investigate Cochlea Metabolism
  96:3, p. 8 - 8a 2009
• Applied Optics
  Ekpenyong Andrew E., Posey Carolyn L., Chaput Joy L., Burkart Anya K., Marquardt Meg M., Smith Timothy J., Nichols Michael G., Determination of cell elasticity through hybrid ray optics and continuum mechanics modeling of cell deformation in the optical stretcher
  48:32, p. 6344 - 6354 2009
• Journal of Biomedical Optics
  Tiede Leann M., Rocha-Sanchez Sonia M., Hallworth Richard, Nichols Michael G., Beisel Kirk, Determination of hair cell metabolic state in isolated cochlear preparations by two-photon microscopy
  12:2 2007
• Brain Research Bulletin
  Matei V. A., Nichols Michael G., Fritzsch Bernd, Beisel Kirk W., Feng F., Pauley S., Near-infrared laser illumination transforms the fluorescence absorbing X-Gal reaction product BCI into a transparent, yet brightly fluorescent substance
  70:1, p. 33 - 43 2006
• Brain Research
  Hallworth Richard J., Currall B., Nichols Michael G., Wu X., Zuo J., Studying inner ear protein-protein interactions using FRET and FLIM
  1091:1, p. 122 - 131 2006
• Photochemistry and Photobiology
  Tiede Le Ann M., Nichols Michael G., Photobleaching of reduced nicotinamide adenine dinucleotide and the development of highly fluorescent lesions in rat basophilic leukemia cells during multiphoton microscopy
  82:3, p. 656 - 664 2006
• Photochemistry and Photobiology
  Barth Erin E., Nichols Michael G., Hallworth Richard, A comparison of the sensitivity of photodamage assays in rat basophilic leukemia cells
  81:3, p. 556 - 562 2005
• Photochemistry and Photobiology
  Barth Erin E., Hallworth Richard J., Nichols Michael G., A Comparison of the Sensitivity of Photodamage Assays in Rat Basophilic Leukemia Cells
  81:3, p. 556 - 562 2005
• Photochemistry and Photobiology
  Nichols Michael G., Barth Erin E., Nichols Jennifer A., Reduction in DNA synthesis during two-photon microscopy of intrinsic reduced nicotinamide adenine dinucleotide fluorescence
  81:2, p. 259 - 269 2005
• Review of Scientific Instruments
  Bigelow Chad E., Mitra Soumya, Harkrider Curtis J., Conover David L., Foster Thomas H., Georgakoudi Irene, Nichols Michael G., Rajadhyaksha Milind, Retrofitted confocal laser scanner for a commercial inverted fluorescence microscope
  72:8, p. 3407 - 3410 2001
• Journal of Pharmaceutical Sciences
  Indig Guilherme L., Bartlett Jeremy A., Anderson Gregory S., Nichols Michael G., Mellon William S., Sieber Fritz, Effect of molecular structure on the performance of triarylmethane dyes as therapeutic agents for photochemical purging of autologous bone marrow grafts from residual tumor cells
  89:1, p. 88 - 99 2000
• Physics in Medicine and Biology
  Hull Edward L., Nichols Michael G., Foster Thomas H., Quantitative broadband near-infrared spectroscopy of tissue-simulating phantoms containing erythrocytes
  43:11, p. 3381 - 3404 1998
• Applied Optics
  Hull Edward L., Nichols Michael G., Foster Thomas H., Localization of luminescent inhomogeneities in turbid media with spatially resolved measurements of cw diffuse luminescence emittance
  37:13, p. 2755 - 2765 1998
• Applied Optics
  Nichols Michael G., Hull Edward L., Foster Thomas H., Design and testing of a white-light, steady-state diffuse reflectance spectrometer for determination of optical properties of highly scattering systems
  36:1, p. 93 - 104 1997
• Photochemistry and Photobiology
  Georgakoudi Irene, Nichols Michael G., Foster Thomas H., The Mechanism of Photofrin® Photobleaching and Its Consequences for Photodynamic Dosimetry
  65:1, p. 135 - 144 1997
• Physics in Medicine and Biology
  Nichols M. G., Foster T. H., Oxygen diffusion and reaction kinetics in the photodynamic therapy of multicell tumour spheroids
  39:12, p. 2161 - 2181 1994
• Cancer Research
  Foster Thomas H., Hartley Donna F., Nichols Michael G., Hilf Russell, Fluence Rate Effects in Photodynamic Therapy of Multicell Tumor Spheroids
  53:6, p. 1249 - 1254 1993

Grants

• AVT Core: Component for Institution # P2500069/Sponsor: National Institute of General Medical Sciences/NIGMS

• Auditory and Vestibular Technology Core/Sponsor: National Institutes of Health/NIH

• Optical Monitoring of Metabolic and Tissue Architecture Changes in the Progression of Skin Cancer/Sponsor: National Institutes of Health/NIH

• IBIF Core Laboratory Equipment/Sponsor: State of Nebraska - LB692

• INBRE: Imaging Core Facility at Creighton University/Sponsor: University of Nebraska Medical Center/UNMC

• The Role of LPA1 in the Etiology of Periodontal Disease/Sponsor: National Institutes of Health/NIH

• Safety Assessment of Translational Forces and Torques on Post-Surgical Epicardial Leads During MRI/Sponsor: Dr. George F. Haddix President's Faculty Research Grant

• IBIF Med Dean Confocal Core Manager Supplemental Funding/Sponsor: State of Nebraska - LB692

• INBRE: Optical Monitoring of Metabolic and Tissue Architecture Changes in the Progression of Skin Cancer/Sponsor: University of Nebraska Medical Center/UNMC

• INBRE: Non-Linear Optical Biopsy for Cancer/Sponsor: University of Nebraska Medical Center/UNMC

• INBRE: Imaging Core Facility at Creighton University/Sponsor: University of Nebraska Medical Center/UNMC

• Physiology and Technology Core/Sponsor: National Institutes of Health/NIH