Catherine A . Opere, BPharm, MBA, PhD

Professor

Pharmacy

C Opere

Contact

School of Pharmacy and Health Professions
Pharmacy
Pharmacy Faculty
Pharmacy Sciences
Graduate School
Pharmaceutical Sciences (Master of Science)
Faculty - Pharmacy, OT, PT
HLSB - Hixson Lied Science Building - 168

Catherine A . Opere, BPharm, MBA, PhD

Professor

Pharmacy

Research Focus

Oxidative stress (OS) is an underlying pathology in retinal neurodegenerative diseases such as age-related macular degeneration, diabetic retinopathy and glaucoma (Dreyer EB. J. Glaucoma. 7(1):62-67). These ocular retinopathies account for a large number of cases of blindness and visual impairment among the elderly in the US, warranting research into potential therapeutic options to prevent visual impairment (Vision Problems in the U.S. Prevalence of Adult Vision Impairment and Age-Related Eye Disease in America. Prevent Blindness America. http://www.visionproblemsus.org/introduction.html). My research focus is directed towards identifying the underlying mechanisms by which OS interacts with pharmacological receptors in ocular tissues, with the aim of revealing potential pharmacological targets that can be manipulated for therapeutic interventions. My specific areas of interests include studies on the regulation of uveal sympathetic and retinal excitatory neurotransmission by OS and OS-metabolites of long chain polyunsaturated fatty acids (PUFAs).  Other areas of study include the regulation of retinal ganglion cells and neural tissue survival by OS-PUFA metabolites; regulation of neurotransmitter release, intraocular pressure and aqueous humor dynamics by hydrogen sulfide donors; development of innovative, slow release hydrogen sulfide donor formulations that can achieve sustainable concentrations in the anterior and posterior segments of the eye for management of glaucoma and other retinopathies; identification and characterization of subtypes of prejunctional (autoreceptors and heteroreceptors) receptors in the mammalian anterior uvea and retina. Receptors of interest include those for glutamate, histamine, prostanoids and cannabinoids.
 

Department

Pharmacy Sciences

Position

Professor

Presentations

  • Singh N, Opere CA, Singh S. Developing a novel lipid-based system of diallyl trisulfide (DATS) for sustained delivery of hydrogen sulfide for the treatment of glaucoma.2020 American Association of Pharmaceutical Scientists, October 25-28, 2020. New Orleans, LA, USA. 2020
  • From bench to bedside: the drug discovery process. Graduate student seminar series MPS 793. Zoom Meeting. 2020
  • Developing an efficient ocular delivery system for the hydrogen sulfide releasing compound, diallyl trisulfide. Singh N, Opere CA, Singh S.2020 American Society for Pharmacology and Experimental Therapeutics. San Diego, CA. 2020
  • Blanchard S, Opere CA.Integration of Pharmacology into an Occupational Therapy Health Conditions Course. Workshop proposal accepted. 2020 Annual AOTA meeting, March 27-30. Boston, MA. 2020
  • Developing a novel lipid-based system of diallyl trisulfide for sustained delivery of hydrogen sulfide for the treatment of Glaucoma. Singh N, Opere CA, Singh S.Midwest Student Biomedical Research Forum, February 2020. Creighton University, Omaha, NE 2020
  • Regulation of Cataractogenesis by Hydrogen Sulfide-NSAID Hybrid Compounds in Cultured Bovine Lenses. Opere CA 2020
  • Ohia SE, Ya Fatou NM, Mitchell L, Robinson J, Ngele K, Opere CA. Regulation of Endogenous Hydrogen Sulfide Concentrations by Cannabinoid in Bovine Isolated Retina. 2020 ARVO Meeting. Baltimore, Maryland. 2020
  • Opere CA, Bankhele P, Salvi A, Jamil J, Munt D, Njie-Mbye YF, Chitnis M, Ohia SE. "Comparative inhibition of neurotransmission by N-acetylcysteine and L-cysteine in bovine isolated retina." Presented at the: Association for Research in Vision and Ophthalmology Annual Meeting, abstract #1886, Orlando, FL May 4-8, 2014. 2014
  • Njie-Mbye YF, Robinson J, Ezeudu C, Mitchell L, Chitnis MK, Opere CA, Ohia SE. "Effect of hydrogen sulfide releasing compounds on aqueous humor dynamics." Presented at the: Association for Research in Vision and Ophthalmology Annual Meeting, abstract #2890, Orlando, FL May 4-8, 2014. 2014
  • Ohia SE, Chitnis MK, Njie-Mbye YF, Robinson J, Ezeudu C, Mitchell L, Opere CA, Whiteman M. "Effect of novel hydrogen sulfide donors (AP67 and AP72) on isolated bovine ciliary artery." Presented at the: Association for Research in Vision and Ophthalmology Annual Meeting, abstract #4348, Orlando, FL May 4-8, 2014. 2014
  • Chitnis MK, Belford R, Robinson J, Mitchell L, Njie-Mbye YF, Opere CA, Ohia SE. "Interaction between hydrogen sulfide and nitric oxide in isolated bovine retina." Presented at the: Experimental Biology meeting, abstract #1060.2, San Diego, CA April 26-30, 2014. 2014
  • Robinson J, Ezeudu C, Mitchell L, Chitnis MK, Opere CA, Ohia SE, Njie-Mbye YF. "Involvement of KATP channels in hydrogen sulfide-induced increase in aqueous humor outflow." Presented at the: Experimental Biology meeting, abstract #1060.3, San Diego, CA April 26-30, 2014. 2014
  • Opere CA, Bankhele P, Salvi A, Jamil J, Munt D, Njie Mbye YF, Chitnis M, Ohia SE. "Comparative pharmacological actions of N-acetylcysteine and L-cysteine on excitatory neurotransmission in isolated bovine retina." Presented at the: Experimental Biology meeting, abstract #1060.4, San Diego, CA April 26-30, 2014. 2014
  • Opere CA, Salvi A, Bankhele P, Jamil J, Njie-Mbye YF, Kulkarni K, Ohia SE. "Inhibitory action of hydrogen sulfide donor, ACS 67 on sympathetic neurotransmitter release in isolated bovine iris-ciliary bodies." Presented at the: ISER symposium, abstract #15, Sarasota, Fl, Sept 29-Oct 2, 2013. 2013
  • Njie-Mbye YF, Robinson J, Kulkarni M, Opere CA, Ohia SE. "Hydrogen sulfide and aqueous humor outflow." Presented at the: ISER symposium, Abstract #4, Sarasota, Fl, Sept 29-Oct 2, 2013. 2013
  • Jamil J, Kegey E, Flowers AF, Flyod NJ, Salvi A, Bankhele P, Guy A, Galano JM, Durand T, Njie-Mbye YF, Kulkarni M, Ohia SE, Opere CA. "Further studies on the role of arachidonic acid metabolites in the regulation of potassium-induced [3H]D-aspartate release from isolated bovine retinae by 5-epi-5-F3t-isoprostane." Presented at the: Association for Research in Vision and Ophthalmology Annual Meeting. ID: 1616232; abstract #6315; Seattle, WA, May 5-9, 2013. 2013
  • Bankhele P, Jamal J, Salvi A, Njie Mbye YF, Kulkarni M, Ohia SE, Opere CA. "Further studies on the inhibition of [3H]D-aspartate release by hydrogen sulfide donors in isolated bovine retina." Presented at the: Association for Research in Vision and Ophthalmology Annual Meeting, Abstract #6325, Seattle, WA, May 5-9, 2013. 2013
  • Salvi A, Bankhele P, Jamal J, Njie Mbye YF, Kulkarni M, Ohia SE, Opere CA. "Regulation of mammalian sympathetic neurotransmitter release and intraocular pressure by hydrogen sulfide donor, GYY 4137." Presented at the: Association for Research in Vision and Ophthalmology Annual Meeting, ID: 1615348; abstract #1976, Seattle, WA, May 5-9, 2013. 2013
  • Njie-Mbye YF, Robinson J, Okpobiri A, Kulkarni M, Opere CA, Ohia SE. "Effect of NaHS, a fast-releasing hydrogen sulfide donor on aqueous humor outflow in porcine trabecular meshwork." Presented at the: Association for Research in Vision and Ophthalmology Annual Meeting, ID 1616794; abstract #3563; Seattle, WA, May 5-9, 2013. 2013
  • Ohia SE, Okpobiri A, Njie-Mbye YF, Robinson J, Kulkarni M, Opere CA. "Pharmacological actions of L-cysteine on isolated bovine ciliary muscles." Presented at the: Association for Research in Vision and Ophthalmology Annual Meeting, ID 1616651, abstract #787; Seattle, WA, May 5-9, 2013. 2013
  • Opere CA, Jamil J, Salvi A, Bankhele P, Flowers AF, Floyd N, Durand T, Galano JM, Guy A, Oger C, Njie-Mbye YF, Kulkarni K, Ohia SE. "Regulation of potassium-induced [3H]D-aspartate release from isolated bovine retina by 5-epi-5-F3t-isoprostane: Role of prostanoid receptors." Presented at the: Experimental Biology meeting, Abstract #7268, Boston, MA April 20-24, 2013. 2013
  • Robinson J, Njie-Mbye YF, Okpobiri A, Kulkarni M, Opere CA, Ohia SE. "Hydrogen sulfide increases aqueous humor outflow in porcine trabecular meshwork." Presented at the: Experimental Biology meeting, Abstract #5025, Boston, MA April 20-24, 2013. 2013
  • Okpobiri A, Njie-Mbye YF, Robinson J, Kulkarni M, Opere CA, Ohia SE. "Effect of hydrogen sulfide on isolated bovine ocular smooth muscle." Presented at the: Experimental Biology meeting, Abstract #5534, Boston, MA April 20-24, 2013. 2013
  • Salvi A, Bankhele P, Jamal J, Njie-Mbye YF, Kulkarni M, Ohia SE, Opere CA. "Regulation of mammalian sympathetic neurotransmitter release and intraocular pressure by hydrogen sulfide donor, GYY 4137." Presented at the: Creighton University St. Alberts Day, Abstract #81, Harper Center, Creighton University, Omaha, NE, Mar 26, 2013. 2013
  • Bankhele P, Jamil J, Salvi A, Opere CA. "Further studies on the Inhibition of [3H]D-aspartate release by hydrogen sulfide donors in isolated bovine retina." Presented at the: Creighton University St. Alberts Day, Abstract #83, Harper Center, Creighton University, Omaha, NE, Mar 26, 2013. 2013
  • Opere CA, Flowers A, Floyd N, Kegey E, Jamil J, Durand T, Galano JM, Guy A, Oger C, Njie-Mbye YF, Ohia SE. "Enhancement of excitatory neurotransmitter release by metabolites of long chain polyunsaturated fatty acids in bovine retina, in vitro." Presented at the: Fourth European Workshop on Lipid Mediators, Pasteur Institute - Paris, September 27-28, 2012. 2012
  • Opere CA, Jamil J, Salvi A, Bankhele P, Njie-Mbye YF, Kulkarni M, Ohia SE. "Inhibition of [3H]D-aspartate release by hydrogen sulfide in isolated bovine retina." Presented at the: Second International Conference on H2S Biology and Medicine, Abstract #P110, Atlanta, Georgia, Sept 20-22, 2012. 2012
  • Njie-Mbye YF, Kulkarni M, Opere CA, Ohia SE. "Hydrogen Sulfide: Endogenous Production and Regulatory Role in Ocular Tissues." Presented at the: Second International Conference on H2S Biology and Medicine, Abstract #P41, Atlanta, Georgia, Sept 20-22, 2012. 2012
  • Opere CA, Flowers A, Jamil J, Kegey E, Floyd N, Salvi A, Bankhele A, Durand T, Galano JM, Guy A, Oger C. "Regulation of excitatory neurotransmitter release by metabolites of long chain polyunsaturated fatty acids in bovine retina, in vitro." Presented at the: Frontiers in Lipid Biology, American Society of Biochemistry and Molecular Biology (ASMB); International Conference on the Bioscience of Lipids (ICBL) and The Canadian Lipoprotein Conference (CLC), Abstract # 2374, Banff Alberta, Canada, September 5-8, 2012. 2012
  • Chitnis MK, Njie-Mbye YF, Opere CA, Ohia SE. "Hydrogen sulfide-induced increase in cyclic AMP production in RPE-J cells: Role of prostanoids, adenyl cyclase and KATP channels." Presented at the: ISER meeting, Berlin, Germany, July 21-25, 2012. Abstract #P061 2012
  • Opere CA, Flowers A, Floyd N, Kegey E, Jamil J, Durand T, Galano JM, Guy A, Oger C, Njie-Mbye YF, Ohia SE. "Role of prostanoid receptors in the excitatory effect of neuroprostanes on potassium induced [3H]D-aspartate release in isolated bovine retina." Presented at the : 19me journe IRPF de Chimie Sorze, France, 15 Juin 2012. 2012
  • Kulkarni M, Njie-Mbye YF, Opere CA, Whiteman M, Ohia SE. "Effect of slow releasing hydrogen sulfide donor GYY4137 on isolated bovine ciliary artery." Presented at the: Association for Research in Vision and Ophthalmology Annual Meeting, Abstracts March 26, 2012 53:6866. May 6-9, 2012, Ft. Lauderdale, Fl. 2012
  • Opere CA, Flowers AF, Floyd N, Kegey E, Jamil J, Durand T, Galano JM, Guy A, Oger C, Njie-Mbye YF, Ohia SE. "Role of prostanoid receptors in the excitatory effect of neuroprostanes on potassium induced [3H]D-aspartate release in isolated bovine retina." Presented at the: Association for Research in Vision and Ophthalmology Annual Meeting, Abstracts March 26, 2012 53:5386. May 6-9, 2012, Ft. Lauderdale, Fl. 2012
  • Flowers AF, Floyd N, Kegey E, Jamil J, Durand T, Galano JM, Guy A, Njie-Mbye YF, Kulkarni K, Ohia SE, Opere CA. "Regulation of K+-induced [3H]D-aspartate release by synthetic neuroprostanes in isolated bovine retina." Presented at the: The Experimental Biology meeting, Journal. 2012;26:843.4. 2012
  • Stroud DD, Kulkarni M, Njie-Mbye YF, Opere CA, Ohia SE. "Pharmacological actions of L-cysteine on isolated bovine irides." Presented at the: The Experimental Biology meeting, Journal. 2012;26:1049.4 2012
  • Kegey E, Jamil JM, Harrison NC, Durand T, Galano JM, Guy A, Njie-Mbye YF, Ohia SE, Opere CA. "Role of prostanoid receptors in the inhibitory effect of 5-F2t-IsoP and its 5-epimer on potassium-induced [3H]D-aspartate release in isolated bovine retina." Presented at the: 8Th GERLI Lipidomics Meeting, Bioactive Lipids, Membranes and Bioactive lipids, Lyon, October, 25-28-2011. 2011
  • Ali H, Opere CA, Singh S. "An easy to use, safe, and efficient spectrophotometric method for the determination of hydrogen sulfide released from its analogues." Presented at the: American Association of Pharmaceutical Scientists, October, 23-27, 2011. Walter E. Washington Convention Center, Washington, D.C. 2011
  • Ali H, Opere CA, Singh S. "Phase-sensitive smart polymer-based sustained release delivery system for hydrogen sulfide analogue." Presented at the: Annual Meeting and Exposition, American Association of Pharmaceutical Scientists, October, 23-27, 2011. Walter E. Washington Convention Center, Washington, D.C. 2011
  • Kegey E, Jamil JM, Harrison NC, Durand T, Galano JM, Guy A, Njie-Mbye YF, Ohia SE, Opere CA. "Role of prostanoid receptors in the inhibitory effect of 5-F2t-IsoP and its 5-epimer on potassium-induced [3H]D-aspartate release in isolated bovine retina." Presented at the: 2nd International conference on Enviromental Stressors in Biology and Medicine Siena, 5-7 October 2011 2011
  • Kulkarni M, Njie-Mbye YF, Okpobiri I, Zhao M, Opere CA, Ohia SE. "Regulation of endogenous hydrogen sulfide production in isolated bovine retina by carbon monoxide and nitric oxide." Program/poster 5669/A581, May 5, 2011, Ft. Lauderdale, Fl. 2011
  • Zhao M, Taylor M, Njie-Mbye YF, Kulkarni M, Opere CA, Ohia SE. "Hydrogen sulfide-induced relaxation of isolated bovine ciliary artery." Program/poster 2658/A139, May 3, 2011, Ft. Lauderdale, Fl. 2011
  • Opere CA, Njie-Mbye YF, Jamil J, Durand T, Galano JM, Guy A, Kulkarni M, Zhao M, Ohia SE. "Regulation of cyclic AMP production in rat RPE-J cells by the synthetic neuroprostane," BA-12high. Program/poster 2067/D1074, May 2, 2011, Ft. Lauderdale, Fl. 2011
  • Opere CA, Jamil JM, Wright A, Durand T, Galano JM, Guy A, Njie-Mbye YF, Ohia SE. "Neuroprostanes inhibit [3H]D-aspartate release in bovine retina, in vitro." Presented at the: Journes CHEVREUL 2011, "LIPIDS & BRAIN 2" Paris 28-30 March 2011. 2011
  • Kegey E, Jamil J, Harrison N, Durand T, Galano JM, Guy A, Njie-Mbye YF, Ohia SE, Opere CA. "Role of prostanoid receptors in the inhibitory effect of synthetic isoprostanes on potassium-induced [3H]D-aspartate release in isolated bovine retina ." Presented at the: Experimental Biology Meeting, March 17, 2011 25:799.4 2011
  • Opere CA, Jamil JM, Wright A, Durand T, Galano JM, Guy A, Njie-Mbye YF, Ohia SE. "Neuroprostanes inhibit [3H]D-aspartate release in bovine retina, in vitro." Presented at the: 7th GERLI Lipodomic Meeting: Lipids in all States, 3-6 October, Anglet-Biarritz, France, 2010. 2010
  • Opere CA, Jamil JM, Wright A, Durand T, Galano JM, Guy A, Njie-Mbye YF, Ohia SE. "Neuroprostanes inhibit [3H]D-aspartate release in bovine retina, in vitro." Presented at the: Third European Workshop on Lipid Mediators, Pasteur Institute - Paris, June 3-4, 2010 2010
  • Ohia SE, Kulkarni M, Njie-Mbye YF, Jackson D, Zhao M, Opere CA. "Measurement of hydrogen sulfide concentration in isolated bovine eyes." Presented at the: Association for Research in Vision and Ophthalmology Annual Meeting, May 2-6, 2010 Abstract #3305/D786. 2010
  • Njie-Mbye YF, Kulkarni M, Jackson D, Zhao M, Opere CA, Ohia SE. "Mechanism of action of hydrogen sulfide on cyclic AMP formation in RPE-J cells." Presented at the: Association for Research in Vision and Ophthalmology Annual Meeting, May 2-6, 2010, Abstract # 3315/D796. 2010
  • Opere CA, Jamil JM, Wright A, Durand T, Galano JM, Guy A, Njie-Mbye YF, Ohia SE. "Regulation of [3H]D-aspartate release by neuroprostanes in bovine retina, in vitro." Presented at the: Association for Research in Vision and Ophthalmology Annual Meeting, May 2-6, 2010, Abstract # 3315/D796. 2010
  • Opere CA, Jamil J, Wright A, Njie-Mbye YF, Ohia SE. "Peroxide-induced production of metabolites of polyunsaturated fatty acids in bovine retina, in vitro." Presented at the: Experimental Biology Meeting, April 6, 2010 24:971.2 2010
  • Njie-Mbye YF, Kulkarni M, Opere CA, Ohia SE. "Hydrogen sulfide stimulates cyclic AMP formation in mammalian retina through the adenylyl cyclase pathway." Presented at the: Experimental Biology Meeting, April 6, 2010 24:962.2 2010