Hollie Siebler, PhD

Assistant Professor

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Contact

College of Arts and Sciences
Biology
EPLY - Eppley Building - 437

Hollie Siebler, PhD

Assistant Professor


I graduated with my Bachelors degree from Dana College in Blair, NE, in 2010. I then finished my Ph.D. in Cancer Research at UNMC in 2015 under Dr. Youri Pavlov. My research was focused on DNA Polymerase Zeta. This is an error-prone DNA polymerase. It specializes in inserting nucleotides across from bulky lesions in template DNA (for example, from harmful UV exposure). This allows cells that would normally die from the damage to survive and replicate. The cost is that Polymerase (Pol) Zeta often makes mutations as it bypasses damage. It is known that Pol Zeta is a major contributor to formation of cancer mutations in result to environmental damage. I was studying the recruitment mechanism of this enigmatic polymerase because it is both friend and foe. I also spent one year as a Visiting Scientist in a breast cancer lab at UNMC studying the protein TSG101 under Dr. Kay Wagner. 

I do not have an active research lab at the moment, but my research interests are in cancer biology and DNA repair. I would like to get involved studing DNA mutations in cancer in human and mouse lines, as well as possibly study the effects of chemotherapy drugs on stems cells and cancer cells.

I also teach as an adjunct professor at UNO teaching Genetics in the summer, in addition to my teaching responsibilities at Creighton and I often serve as a Freshman advisor.
 

Department

Biology

Position

Assistant Professor

Books

  • Methods in enzymology
    Baranovskiy Andrey G, etal Iron-Sulfur Clusters in DNA Polymerases and Primases of Eukaryotes. [Book Chapter] 2018
  • Methods in Enzymology
    Lee Hui-Ting, etal A Thermodynamic Approach for the Targeting of Nucleic Acid Structures Using Their Complementary Single Strands [Book Chapter] 2011

Publications

  • Genes
    Siebler Hollie M, etal DNA Polymerase ζ without the C-Terminus of Catalytic Subunit Rev3 Retains Characteristic Activity, but Alters Mutation Specificity of Ultraviolet Radiation in Yeast
    13:9 2022
  • DNA repair
    Stepchenkova E. I., etal Defect of Fe-S cluster binding by DNA polymerase δ in yeast suppresses UV-induced mutagenesis, but enhances DNA polymerase ζ - dependent spontaneous mutagenesis
    49, p. 60 - 69 2017
  • DNA repair
    Siebler Hollie M., etal A novel variant of DNA polymerase zeta, Rev3 Delta C, highlights differential regulation of Pol32 as a subunit of polymerase delta versus zeta in Saccharomyces cerevisiae
    24, p. 138 - 149 2014
  • Biophysical journal
    Lee Hui-Ting, etal Targeting DNA Hairpin Loops with their Partially Complementary Strands
    98:3, p. 656 - 656a 2010