Garrett A . Soukup, PhD

Professor

Biomedical Sciences

photo placeholder for Soukup, Garrett A

Contact

GarrettSoukup@creighton.edu
School of Medicine
Biomedical Sciences
Graduate School
Biomedical Sciences (Master of Science)
Biomedical Sciences (Doctorate)
Translational Hearing
CRISS I - Criss 1 - 325

Garrett A . Soukup, PhD

Professor

Biomedical Sciences

Garrett Soukup is a biochemist and molecular biologist with a longstanding interest in RNA structure, function, and regulation of gene expression. He received a B.S. in Biology in 1991 from Northwest Missouri State University in Maryville, Missouri. He earned a Ph.D. in 1997 from the University of Nebraska Medical Center in Omaha, Nebraska, working with Dr. L. James Maher III there and at the Mayo Clinic in Rochester, Minnesota. He completed postdoctoral training in 2000 at Yale University in New Haven, Connecticut, working with Dr. Ronald R. Breaker. He joined the faculty at Creighton University School of Medicine in 2000, where he has served various roles in graduate and medical student education and research training. 

 

Research Focus

Garrett Soukup's research interests over his career have included RNA-ligand interactions, mechanisms of RNA catalysis, biological roles of functional RNAs including ribozymes and riboswitches, and regulatory roles of microRNAs in the development and maintenance of mechanosensory cells and sensory neurons important for hearing.

Department

Biomedical Sciences

Position

Professor

Books

  • Soukup Garrett A, Catalytic RNA 2013
  • Catalytic RNA
    Soukup Garrett A, Chapter Four: HDV Family of Self-Cleaving Ribozymes [Book Chapter] 2013
  • Catalytic RNA
    Soukup Garrett A, Chapter Three: A Mechanistic Comparison of the Varkud Satellite and Hairpin Ribozymes [Book Chapter] 2013
  • Catalytic RNA
    Soukup Garrett A, Contributors [Book Chapter] 2013
  • Catalytic RNA
    Soukup Garrett A, Color Plate [Book Chapter] 2013
  • Catalytic RNA
    Soukup Garrett A., Preface [Book Chapter] 2013
  • Catalytic RNA
    Soukup Garrett A, Chapter Five: The Structural and Functional Uniqueness of the glmS Ribozyme [Book Chapter] 2013
  • Catalytic RNA
    Soukup Garrett A, Chapter Two: Bridging the Gap Between Theory and Experiment to Derive a Detailed Understanding of Hammerhead Ribozyme Cata [Book Chapter] 2013
  • Catalytic RNA
    Soukup Garrett A, Chapter One: The Hammerhead Ribozyme: Structure, Catalysis, and Gene Regulation [Book Chapter] 2013
  • Catalytic RNA
    Soukup Garrett A, Chapter Six: The Role of snRNAs in Spliceosomal Catalysis [Book Chapter] 2013
  • Non-Protein Coding RNAs
    Soukup Garrett A., Structure and mechanism of the glmS ribozyme [Book Chapter] 2009
  • Nucleic acid switches and sensors
    Soukup Garrett A., Allosteric ribozymes as molecular switches and sensors [Book Chapter] 2006

Articles

  • McCarthy T. J., etal Ligand Requirements for glmS Ribozyme Self-Cleavage (Erratum) 2006
  • McCarthy Tom J., etal Ligand Requirements for glmS Ribozyme Self-Cleavage (DOI:10.1016/j.chembiol.2005.09.006) 2006

Publications

  • Molecular Neurobiology
    Banks Samantha A., etal Sensational MicroRNAs
    57:1, p. 358 - 371 2020
  • Scientific Reports
    Weston Michael D., etal A mouse model of MIR-96, MIR-182 and MIR-183 misexpression implicates MIRNAs in cochlear cell fate and homeostasis
    8:1 2018
  • Biophysical journal
    Strauss-Soukup Juliane, etal Ornithine Decarboxylase Antizyme Pseudoknot RNA Binding to Spermine Regulates Gene Expression
    114:3, p. 438a - 438a 2018
  • Plos One
    Ebeid Michael, etal Transcriptome-Wide Comparison of the Impact of Atoh1 and miR-183 Family on Pluripotent Stem Cells and Multipotent Otic Progenitor Cells
    12:7, p. E0180855 - E0180855 2017
  • Molecular therapy
    Kim Ju-hyun, etal 139. Controllable Target-Specific Transgene Induction Using an Allosteric trans-Splicing Ribozyme
    23, p. S56 - S57 2015
  • PLoS One
    Zhang Qian, etal Identifying microRNAs involved in aging of the lateral wall of the cochlear duct
    9:11, p. e112857 - e112857 2014
  • Journal of Neuroscience
    Liu H., etal Characterization of transcriptomes of cochlear inner and outer hair cells
    34:33, p. 11085 - 11095 2014
  • Journal of Neuroscience
    Liu Huizhan, etal Characterization of Transcriptomes of Cochlear Inner and Outer Hair Cells
    34:33, p. 11085 - 11095 2014
  • ACS Chemical Biology
    Kim Juhyun, etal Conditional and target-specific transgene induction through RNA replacement using an allosteric trans-splicing ribozyme
    9:11, p. 2491 - 2495 2014
  • Soukup Garrett A., Preface. Catalytic RNA. 2013
  • PloS one
    Zhang Qian, etal Identifying MicroRNAs Involved in Degeneration of the Organ of Corti during Age-Related Hearing Loss
    8:4 2013
  • Nebraska Academy of Sciences, PO Box 880410 Lincoln NE 68588-0410 United States. [np]. Apr 2012
    Holmes Zachariah, etal EFFECT OF MICRORNA-183 FAMILY ON EXPRESSION OF GENES THAT REINFORCE NEUROSENSORY CELL FATE IN THE INNER EAR 2012
  • Indian Journal of Human Genetics
    Barritt Laura C., etal Conditional deletion of the human ortholog gene Dicer1 in Pax2-Cre expression domain impairs orofacial development
    18:3, p. 310 - 319 2012
  • Genesis
    Kersigo Jennifer, etal The role of sensory organs and the forebrain for the development of the craniofacial shape as revealed by Foxg1-cre-mediated microRNA loss
    49:4, p. 326 - 341 2011
  • Developmental Dynamics
    Weston Michael D., etal MicroRNA-183 family expression in hair cell development and requirement of microRNAs for hair cell maintenance and survival
    240:4, p. 808 - 819 2011
  • International Journal for Parasitology
    Gong Ai-Yu, etal MicroRNA-122 controls expression of intercellular adhesion molecule-1 in epithelial cells in response to Cryptosporidium parvum infection
    41:3/4, p. 397 - 403 2011
  • Chembiochem
    Klawuhn K., etal Analysis of Metal Ion Dependence in glmS Ribozyme Self-Cleavage and Coenzyme Binding
    11:18, p. 2567 - 2571 2010
  • Genome Medicine
    Weston Michael D., etal MicroRNAs sound off
    1:6, p. 59 2009
  • Brain Research
    Soukup Garrett A., Little but loud: Small RNAs have a resounding affect on ear development
    1277, p. 104 - 114 2009
  • Developmental Biology
    Soukup Garrett A., etal Residual microRNA expression dictates the extent of inner ear development in conditional Dicer knockout mice
    328:2, p. 328 - 341 2009
  • Journal of Immunology
    Gong Ai Yu, etal MicroRNA-513 regulates B7-H1 translation and is involved in IFN-γ-induced B7-H1 expression in cholangiocytes
    182:3, p. 1325 - 1333 2009
  • Methods in Molecular Biology
    Soukup Garrett A., Identification of metabolite-riboswitch interactions using nucleotide analog interference mapping and suppression
    540, p. 193 - 206 2009
  • Cell and Tissue Research
    Beisel Kirk W., etal Regenerating cochlear hair cells: quo vadis stem cell
    333:3, p. 373 - 379 2008
  • Panminerva Medica
    Pauley S., etal Stem cells and molecular strategies to restore hearing
    50:1, p. 41 - 53 2008
  • Evolution & Development
    Pierce Marsha L., etal MicroRNA-183 family conservation and ciliated neurosensory organ expression
    10:1, p. 106 - 113 2008
  • International Journal of Developmental Biology
    Fritzsch Bernd, etal Molecular evolution of the vertebrate mechanosensory cell and ear
    51:6-7, p. 663 - 678 2007
  • Brain Research
    Weston Michael D., etal MicroRNA gene expression in the mouse inner ear
    1111:1, p. 95 - 104 2006
  • Nature Structural and Molecular Biology
    Jansen Joshua A., etal Backbone and nucleobase contacts to glucosamine-6-phosphate in the glmS ribozyme
    13:6, p. 517 - 523 2006
  • Nucleic Acids Research
    Soukup Garrett A., Core requirements for glmS ribozyme self-cleavage reveal a putative pseudoknot structure
    34:3, p. 968 - 975 2006
  • Chemistry and Biology
    McCarthy Tom J., etal Ligand requirements for glmS ribozyme self-cleavage
    12:11, p. 1221 - 1226 2005
  • Chemistry and Biology
    Soukup Garrett A., Aptamers meet allostery
    11:8, p. 1031 - 1032 2004
  • Current Opinion in Structural Biology
    Soukup Juliane K., etal Riboswitches exert genetic control through metabolite-induced conformational change
    14:3, p. 344 - 349 2004
  • American Journal of PharmacoGenomics
    Steele David, etal Engineered catalytic RNA and DNA
    3:2, p. 131 - 144 2003
  • American Journal of PharmacoGenomics
    Steele David, etal Engineered catalytic RNA and DNA : new biochemical tools for drug discovery and design
    3:2, p. 131 - 144 2003
  • Nucleic Acids Research
    Kertsburg Alexis, etal A versatile communication module for controlling RNA folding and catalysis
    30:21, p. 4599 - 4606 2002
  • Nucleic Acids Research
    Kertsburg Alexis, etal A versatile communication module for controlling RNA folding and catalysis
    30:21, p. 4599 - 4606 2002
  • Nucleic Acids Research
    Jose Antony M., etal Cooperative binding of effectors by an allosteric ribozyme
    29:7, p. 1631 - 1637 2001
  • RNA
    Soukup Garrett A., etal Generating new ligand-binding RNAs by affinity maturation and disintegration of allosteric ribozymes
    7:4, p. 524 - 536 2001
  • Current Opinion in Structural Biology
    Soukup Garrett A., etal Allosteric nucleic acid catalysts
    10:3, p. 318 - 325 2000
  • Journal of Molecular Biology
    Soukup Garrett A., etal Altering molecular recognition of RNA aptamers by allosteric selection
    298:4, p. 623 - 632 2000
  • Trends in Biotechnology
    Soukup Garrett A., etal Nucleic acid molecular switches
    17:12, p. 469 - 476 1999
  • RNA
    Soukup Garrett A., etal Relationship between internucleotide linkage geometry and the stability of RNA
    5:10, p. 1308 - 1325 1999
  • Structure
    Soukup Garrett A., etal Design of allosteric hammerhead ribozymes activated by ligand-induced structure stabilization
    7:7, p. 783 - 791 1999
  • Proceedings of the National Academy of Sciences of the United States of America
    Soukup Garrett A., etal Engineering precision RNA molecular switches
    96:7, p. 3584 - 3589 1999
  • Nucleic acids symposium series
    Koizumi M., etal Allosteric ribozymes sensitive to the second messengers cAMP and cGMP., p. 275 - 276 1999
  • Nature Structural Biology
    Koizumi Makoto, etal Allosteric selection of ribozymes that respond to the second messengers cGMP and cAMP
    6:11, p. 1062 - 1071 1999
  • Nucleic Acids Research
    Soukup Garrett A., etal Selection and characterization of RNAs that relieve transcriptional interference in Escherichia coli
    26:11, p. 2715 - 2722 1998
  • Journal of Molecular Biology
    Soukup Garrett A., etal Selection of RNAs that bind to duplex DNA at neutral pH
    259:2, p. 216 - 228 1996
  • Bioconjugate Chemistry
    Soukup Garrett A., etal Preparation of Oligonucleotide-Biotin Conjugates with Cleavable Linkers
    6:1, p. 135 - 138 1995

Awards

  • 2021 Dedicated Teacher Award
    Creighton University School of Medicine